The advent of molecular genetics has equipped and expanded the realm and scope of genetics as well as any biological sciences. Study of any disease at molecular level makes it easy to device prophylactic, diagnostic and curative methods forit. Population or evolutionary studies as well as individual studies, MAS, breed characterization and improvement have all taken a leap using these techniques. The necessity of molecular tools in research as well as academics makes it mandatory to update the undergraduate and postgraduate students as well as faculty with the basic knowledge of molecular tools. The book has been authored with this aim of disseminating knowledge to the scholars and teachers. It is useful for veterinary as well as animal biotechnology or allied fields.
Dr. Nandani Kumari, Assistant Professor-cum-Junior Scientist, A.H., Animal Genetics and Breeding, Ranchi Veterinary College, BAU). After completing her Ph.D. in Animal Breeding and Genetics from Ranchi Veterinary College, she joined teaching and has a teaching experience as Assistant Professor-cum-Junior Scientist at Deptt. of AGB, RVC of about eight years. She has cleared CSIR NET(AIR 21) AND ICAR NET ( shortlisted for interview of ARS scientist among five for a total of one seat). She has authored five books with ISBNs (two as first author), two manuals, one Hindi book (total=Eight books). A total of 32 research articles, four lead papers, 28 abstracts, sixteen popular articles, and three invited lectures are to her credit till date. She has organized five online Career counselling series called Zenith, one workshop on statistical analysis as organizing secretary, one national conference (IVEF 9th National Conference) as co-organizing secretary, one FDP-21 days as coordinator, and two training programmes on molecular analysis in the molecular genetics Lab of AGB. Currently, she is also the CO-PI of ACRIP on Goat, RVC, Ranchi. With a penchant for writing, she is a part of the editorial board of Pathar Krishi and BAU Research Journal. She has been nominated as Convener, Jharkhand chapter of IVA Lady vet shakti. She has anchored important events like national conferences (IVEF-2022 and IAVP-20), Mahila Goshthi of Agrotech Kisan Mela-2023. She has taken about 80 classes of Framer’s training hitherto and has received several prestigious awards at various esteemed forums. She is a poetess with a book of a poem published by her, as well as a blogger and Anchor with experience in anchoring at various vital events, including inaugural and or valedictory of several conferences, college foundation day programme, world veterinary day programme, Kavi Sammelan, IAS felicitation, etc. She has been awarded with best thesis award and young scientist award along with award for best poster presentation at Global Research Initiatives for Sustainable Agriculture & Allied Sciences, 2022. She has also been awarded for second best poster presentation at IVEF-9th National conference during 4th-5th Nov., 2022. Along with best teacher award at 2019- DISHA and Young Scientist award at ICALVES- 2019, she has also received Venus women of the year award. She is the state representative of Society of Veterinary Science and Biotechnology and member of coveted society like Society for Conservation of Domestic Animal Biodiversity, Indian Society of Genetics Biotechnology Research and Development (ISGBRD), etc.
Dr. Manjari Pandey presently working as Assistant Professor -cum- Junior Scientist in Faculty of Veterinary Sciences and Animal Husbandry, Birsa Agricultural University, Ranchi, Jharkhand. She did her Ph.D. specialisation in Animal Genetics and Breeding as a major subject and Animal Biotechnology and Poultry Science as minor subjects from ICAR- Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P. She got her M.V.Sc. degree in Animal Genetics and Breeding from ICAR- National Dairy Research Institute, Karnal, Haryana in 2018. She earned her B.V.Sc. & A.H. degree from Govind Ballabh Pant University of Agriculture and Technology, Pantnagar in 2016. She is the proud recipient of ICAR- Institute fellowship during her M.V.Sc. program and GoI, DST-INSPIRE fellowship during her Ph.D. program. She has also received Academic Excellence award in 2019 and Young Scientist award in 2022. She has currently a total of 32 publications including 8 research articles published in both national and international journals and 6 book chapters. Apart from these, 17 of her research abstracts are published in the compendiums of different national and international conferences. She has received best oral presentation award in VI International Conference on Global Research Initiatives for Sustainable Agriculture & Allied Sciences, 2022 and best paper presentation award in 3 days International Conference on “Advances in Agricultural, Veterinary and Allied Sciences forImproving Livelihood and Environmental Security, 2022”. She is a lifetime member of prestigious professional societies like Society for conservation of Domestic Animal Biodiversity, Indian Poultry Science Association and Indian Society of Animal Genetics and Breeding.
Dr. Rakesh Kumar was born in Bihar Sharif, which is located in the Nalanda district of Bihar. He has completed his graduation (B.V.Sc & A.H.) from G.B.P.U.A&T (Pantnagar), Uttarakhand, Master’s (M.V.Sc.) from ICARIVRI (ICAR-Indian Veterinary Research Institute), Izzatnagar, Bareilly (U.P.) and Ph.D. from ICAR-NDRI (ICAR-National Dairy Research Institute), Karnal (Haryana). He has been awarded ICAR-SRF and ICMR-JRF during the Ph.D. program. He has worked as Teaching Associate at Ranchi Veterinary College, Jharkhand and Scientist (Animal Science) at Regional Research Station (Punjab Agricultural University), Ballowal Saunkhri, Punjab. Currently, he is working as a Scientist (ARS) in the Division of Livestock and Fishery Management of ICAR-RCER, Patna. His research expertise is on sperm mediated gene transfer in goats and identification of SNP markers related to heat stress, production, and reproduction traits in livestock. His innovative research has resulted in published several papers, popular articles, training manuals, and technical bulletins in the journal of national and international repute.
Dr. Sushil Prasad is Dean & University Professor-cumChief Scientist, Ranchi Veterinary College, BAU, Ranchi, Jharkhand. He has developed a suitable dual type variety of chicken named Jharsim for rural poultry production under backyard system. He has been nationally felicitated recently for the coveted Breed registration of pig (Banda) with Dr. Ravindra Kumar. He has published sixty research articles in National and International journals along with many bulletins and articles in Livestock Production and Management. He has received many awards particularly Fellow award for contribution in Livestock Production and Management in 2015 and distinguished Scientist forrelease of Jharsim Variety of chicken.
1. General Precautions in Any Molecular Biology Lab ........................ 1
2. Preparation of Different Concentrations of Workable Solutions from Stock Solutions ................... 5
3. Working of Basic Equipments in Laboratory .................................... 9
4. Procedure for Extraction of DNA from Blood ................................ 13
5. Extraction of Mitochondrial DNA ..................................................... 17
6. Extraction of DNA from Sperm Cell ................................................. 19
7. RNA Isolation from Blood Sample .................................................... 23
8. RNA Extraction from Tissue Sample ................................................. 25
9. Plasmid Isolation ................................................................................... 27
10. Preparation of Agarose Gel for Unamplified (Raw) DNA and Amplified (PCR Product) DNA ...... 31
11. Karyotyping of Animal Blood (Goat in Current Case) .................. 35
12. Diploid Chromosome Number in Different Species ....................... 41
13. Fluorescent in-situ Hybridization Technique and Chromosome Painting ................... 43
14. PCR Primer Designing.......................................................................... 47
15. Procedure/Steps of Polymerase Chain Reaction (PCR) ................. 51
16. Microsatellite-Primed PCR .................................................................. 57
17. PCR-SSCP Analysis of Target Gene Fragments ............................... 61
18. Synthesis of cDNA ................................................................................ 65
19. Real Time Quantitative PCR................................................................ 67
20. Marker and Its Type ............................................................................. 71
21. Protein Extraction ................................................................................. 75
22. SDS- PAGE Protocol ............................................................................. 79
23. To Study about Types of RNAs and their Function ....................... 83
24. DNA and Its Types ............................................................................. 91
25. Major Types of Microscope ................................................................ 97
